HYDROKAT® Multiplex qPCR – Legionella
HYDROKAT® Multiplex qPCR – Legionella: A molecular method for the multiplex confirmation and discrimination of Legionella spp., L. pneumophila, L. pneumophila serogroup 1 and L. pneumophila serogroup 2–14.
The method has been validated as an alternative confirmation method according to EN ISO 11731 Sec. 8.5, EN ISO 16140 – 6 and DIN 38411-25
Product Features
- Confirm suspicious colonies within one hour – Reduce your analytical time to result by up to three days
- Reliable detection of recalcitrant Legionella anisa in Legionella spp. channel
- Hot start real time PCR
- 100% inclusivity: more than 200 Legionella strains tested with correct PCR positive results
- 100% exclusivity: more than 100 non-Legionella strains tested with correct PCR negative results
- Very robust freeze dried MasterMix optimized for multiplexing purpose and reproducible PCR results
- Validated for thermal cyclers from different suppliers
Application – Instructions for use
Pick a suspicious colony from the selective legionella culture medium (e.g. BCYE+AB Agar or GVPC agar, etc.).
Resuspend in PCR grade water.
Lyse bacteria by boiling for approx. 5 minutes, for example, using a thermomixer.
Transfer 20 ml of the lysate to a single tube of PCR grade water
Run PCR protocol on thermal cycler.
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